Best Answer: It's when you use several steps in a series of dilutions instead of just doing one big dilution. For instance say you have a 10M solution (it doesn't matter what) and you want to make a 10mM solution (.01M). If you did this in one step you would need to take 1 mL of the 10M solution and put it into 999 mL of your solvent. Instead you could do a serial dilution: Take 1 mL 10M solution, put it in 9mL solvent to make a 1M solution. To perform a serial dilution, a small amount of a well-mixed solution is transferred into a new container and additional water or other solvent * is added to dilute the original solution. Next take 1 mL of the 1M solution and put it in 9 mL solvent (0.1M solution). Finally, 1 mL of the 0.1 solution and put it in 9mL solvent to get the solution you wanted. Using a serial dilution you get the same final concentration, but now you only used 27 mL of solvent instead of 999 mL. Aside from saving solvent, other reasons this might be good include: - Available glassware, perhaps you have a lot of 10mL, 50mL, and 100mL volumetric flasks in your lab, but not a lot of 1000mL flasks, the serial dilution may be the only way to get the concentration you want. - Stability of different concentrations. Say you are making standards for calibration of an instrument. You might actually use the low level for the test you run, but the shelf life of a low concentration standard is shorter than that of a higher concentration standard. You might replace the dilute one (.01 M in the example) every day, the intermediate one (0.1M) every 2 weeks, and the concentrated one (1M) every 2 months. In the end it make the standard preparation that much easier if you have the two middle concentrations. There are others, for instance if you're doing a calibration curve, or something like that, but hopefully this explains the issue somewhat. Serial dilutions is diluting a solution by half each time. So you have 50 ml of Solution A. Then you take 25 mL of that solution and put it in another 25 mL of water. Then from THAT take 25mL and add it to another 25mL of water. The concentration of Solution A is being halved each time you are diluting it. There are many practical uses for serial dilutions. Concentration is correlated to absorbance of light. The higher the concentration the higher percent of light it absorbs. Spectrometers measure this and concentrations can be determined.
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